Myocardial mast cells degranulation during cardiac surgery is not cardioplegic solution temperature - dependent

Mircea Crîsnic¹, Petre Deutsch¹, Horia Cioloca¹, Marius Sintean¹, Mioara Cocora², Dan Nechifor², Ion Socoteanu², Marius Raica³.
1. Secţia ATI, Institutul de Boli Cardiovasculare. 2. Secţia Chirurgie Cardiovasculară I, Institutul de Boli Cardiovasculare Timişoara; 3. Disciplina de Histologie, Universitatea de Medicină şi Farmacie „Victor Babeş”, Timişoara

Abstract

Background. Recent studies suggest that normothermic myocardial protection during heart surgery is preferable against hypothermic cardio-protection. Myocardial mast cells were recently involved in ischemia-reperfusion injuries. The present study tries to evaluate the effect of cardioplegic solution temperature on myocardial mast cells degranulation.

Patients and method. We included 13 patients, distributed in the hypothermic group (n=7) in which we administered cardioplegic solution at 12-15°C, and the normothermic group (n=6), with normothermic cardioplegic solution. From all the patients we took atrial tissue samples before aortic crossclamping, before aortic unclamping and after reperfusion. Alcian blue/safranin staining was used to study the mast cells.

Results. Ischemia-reperfusion induced mast cells degranulation was important in our study. Degranulation of alcian-stained mast cells occured mainly during aortic crossclamping and that of safranin-stained mast cells continued also during reperfusion. There were no differences betwen the two groups.

Conclusions. Myocardial mast cells degranulation during heart surgery performed with cardiopulmonary bypass is not temperature-dependent.

Key words: cardiac surgical procedures, cardiopulmonary bypass, mast cells, cardioplegic solutions

Degranularea mastocitelor cardiace în timpul circulaţiei extracorporeale nu depinde de temperatura soluţiei de cardioplegie

Jurnalul Român de Anestezie Terapie intensivă 2005 Vol.12 Nr.1, 14-19